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Supplementation with fruit and vegetable extracts may decrease dna damage in the peripheral lymphocytes of an elderly population
Smith MJ et al., Nutrition Research, Vol.19, No.10, Oct. 1999, pp. 1507–1518
Oxidativer Stress mit vermehrter Freisetzung von freien Sauerstoffradikalen führt zu Schäden, auch an der DNS. Dadurch ist oxidativer Stress auch ursächlich an der Pathogenese von maligner Entartungen beteiligt. Unter Supplementation der Obst- und Gemüsekonzentrate über 80 Tage wurden DNS-Brüche, und damit das Ausmaß der Schädigung durch freie Radikale, mittels des so genannten COMET-Assay in peripheren Lymphozyten gemessen. Eine hoch signifikante Abnahme von DNS-Brüchen (p < 0,0001) um durchschnittlich 67% konnte dokumentiert werden, wobei die Resultate weder geschlechts- noch raucherspezifische Unterschiede aufwiesen.
NUTITION RESEARCH, VOL. 19. NO. 10, PP. 1507 – 1518, 1999
Micah J. Smith B.S1., Paula F. Inserra Ph.D2.,Ronald R. Watson Ph.D.3, John A. Wise Ph.D.4, and Kim L. O’Neill Ph.D1.
1Department of Microbiology, Brigham Young University, Provo, UT 84602;
2Arizona Cancer Cernter, University of Aizona, Tucson, AZ 85724;
3Arizona Prevention Center, University of Arizona, Tucson, AZ 85724-5155,
4Natural Alternatives International, San Marcos, CA 92069.
Abstract (engl.)
Fruit and vegetable consumption has been heralded for its ability to decrease the overall risk of developing cancer and other diseases. Mounting evidence supports the beneficial nature of anti- oxidants, carotenoids and other phytonutrients found in fruits and vegetables. One proposed mechanism of antioxidant protection is the shielding of cellular DNA from oxidative damage and therefore mutations. This may be especially helpful in older populations. We tested the concept that a daily regimen of supplementation with fruit and vegetable extracts (Juice Plus+®) would reduce the amount of DNA damage in the peripheral lymphocytes of the elderly. In a blind study, a group of twenty elderly volunteers (mean age = 68) were given supplements twice daily for 80 days with blood samples drawn before and after intervention. These samples were compared using the comet assay, a technique that quantifies DNA damage to individual nuclei. Each sample was tested in triplicate, and tail moment data was collected from over 200 comets per sample. Paired t-test analysis revealed a highly significant (p <0.0001) decrease in measured DNA damage between pre (13.24±2.77) and post (4.41± 2.76) treatment tail moment. Screening of test subjects' personal data showed no apparent relationship between age, sex, or smoking. In this initial study, we conclude that a daily course of fruit and vegetable extract supplementation may reduce the level of DNA damage found in the peripheral lymphocytes of seniors.
© 1999 Elsevier Science Inc.
